Cloning of variable region and signal peptide genes of anti-cd20 monoclonal antibody by rlm-race 20單克隆抗體可變區(qū)基因及其信號肽基因
This paper studied the cloning signal peptide and promoter of grb-ast gene 本論文對grb-ast基因的信號肽序列及其啟動子進行分離克隆的研究。
In addition, the function of secreting protein by grb-ast signal peptide was also studied in this paper 此外,還探討了grb-ast信號肽分泌蛋白的功能。
Computer-assisted analysis revealed there was a signal peptide near the n-terminal of this protein 軟件預測0rf132有一個信號肽序列(signalpeptide),最可能的切割位點在和r20之間。
Sequence analysis shows that the n-terrninal 1-25 amino acid sequence is a predicted signal peptide 序列分析也顯示蛋白n?端具有信號肽序列特征,表明ecbp21可能是一種分泌到細胞外的蛋白。
Our data indicate that in the insect cells, we can express the human full-length gene carrying the introns and signal peptide 我們的結果表明在昆蟲細胞中能夠正確的表達帶有自身內(nèi)含子和信號肽的人源蛋白基因。
The main results are the following : 1 . the hplap without signal peptide was expressed in p . partoris smd1 168 by the ppicz-a a plasmid . 2 在畢赤酵母幾psrtorjssmdi168e株中用表達載體ppiczqa成功表達了去掉信號肽的有活性的堿性磷酸酯酶hplap。
Hydrophobicity analysis suggested that the protein was highly hydrophilic, especialy at the first 24 amino-acid, this region could be function as signal peptide 將此基因重組到表達質(zhì)粒載體pgex-4t-1上,轉化到大腸桿菌bl21(de3)中,經(jīng)iptg誘導后,可得到高效表達。
In this paper, signal peptide was added before the n-terminus of cema with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity 本文通過在cema的n端添加信號肽,對其進行改造,以探索不降低抑菌作用的同時,降低其對植物細胞毒害作用的可能性。
In this thesis, signal peptide analyse software and trans-menbrane helix analyse software were integrated in the research of mycobacterium tuberculosis ( mtb ) secreted proteins prediction 本研究嘗試了利用信號肽預測軟件和跨膜螺旋預測軟件對結核桿菌全蛋白組進行分泌性蛋白的預測分析研究。